rabbit anti phospho c fos (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc rabbit anti phospho c fos
Rabbit Anti Phospho C Fos, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+phospho+c+fos/bio_rxiv__64898__2026__04__02__716115-150-24-27
Average 86 stars, based on 1 article reviews

rabbit anti phospho c fos - by Bioz Stars, 2026-10

86/100 stars

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Incubation:

Article Title: An inhibitory circuit from central amygdala to zona incerta drives pain-related behaviors in mice
Article Snippet: Sections were rinsed in PBS, incubated in 0.1% Triton X- 100 in PBS for 10 min at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X- 100, 0.05% Tween- 20 and 1% bovine serum albumin (BSA) for 30 min at room temperature. .. Sections were then incubated for 72 hr at 4 °C in mouse anti- PKCδ (1:1000, BD Biosciences, 610397), rabbit anti- Phospho- c- Fos (1:2000, Cell Signaling Technology, 5348) or rat anti- mCherry (1:500, Invitrogen, M11217) in 1.5% NGS blocking solution with 0.1% Triton X- 100, 0.05% Tween- 20 and 1% BSA. .. Sections were then rinsed in PBS and incubated in Alexa Fluor 647- conjugated goat anti- mouse (1:100, Invitrogen, A21235), Alexa Fluor 647- conjugated goat anti- rabbit (1:250, Invitrogen, A21244), or goat anti- rat Cy3 (1:250, Invitrogen, A10522) secondary antibodies in 1.5% NGS blocking solution with 0.1% Triton X- 100, 0.05% Tween 20 and 1% BSA, protected from light, for 2 hr at room temperature.

Article Title: Neuronal Correlates of Hyperalgesia and Somatic Signs of Heroin Withdrawal in Male and Female Mice
Article Snippet: .. We incubated the sections overnight at 4°C with rabbit anti-phospho-c-Fos (1:2000; catalog #5348; Cell Signaling Technology) in blocking solution. .. After rinsing with PB three times for 10 min each, we incubated the sections for 1 h at room temperature with biotinylated goat anti-rabbit antibody (1:200; catalog #PK6101; Vector Laboratories) in blocking solution.

Article Title: An inhibitory circuit from central amygdala to zona incerta drives pain-related behaviors in mice
Article Snippet: Sections were rinsed in PBS, incubated in 0.1% Triton X-100 in PBS for 10 min at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100, 0.05% Tween-20 and 1% bovine serum albumin (BSA) for 30 min at room temperature. .. Sections were then incubated for 72 hr at 4 °C in mouse anti-PKCδ (1:1000, BD Biosciences, 610397), rabbit anti-Phospho-c-Fos (1:2000, Cell Signaling Technology, 5348) or rat anti-mCherry (1:500, Invitrogen, M11217) in 1.5% NGS blocking solution with 0.1% Triton X-100, 0.05% Tween-20 and 1% BSA. .. Sections were then rinsed in PBS and incubated in Alexa Fluor 647-conjugated goat anti-mouse (1:100, Invitrogen, A21235), Alexa Fluor 647-conjugated goat anti-rabbit (1:250, Invitrogen, A21244), or goat anti-rat Cy3 (1:250, Invitrogen, A10522) secondary antibodies in 1.5% NGS blocking solution with 0.1% Triton X-100, 0.05% Tween 20 and 1% BSA, protected from light, for 2 hr at room temperature.

Article Title: CGRP receptor-expressing neurons in the central amygdala contributes to injury-induced pain hypersensitivity
Article Snippet: To prevent nonspecific binding, the sections were blocked in a solution containing 5 % normal goat serum (NGS) (Vector Labs, Burlingame, CA), 0.1 % Triton X-100, 0.05 % Tween-20, and 1 % bovine serum albumin (BSA) for 30 minutes at room temperature. .. Subsequently, the sections were incubated for 72 hours at 4°C with specific primary antibodies for each experiment, including mouse anti-PKCδ (1:1000, BD Biosciences, 610397), rabbit anti-Phospho-c-Fos (1:2000, Cell Signaling Technology, 5348), rat anti-mCherry (1:500, Invitrogen, M11217) and rabbit anti-Phospho-p44/42 MAPK (Erk1/2) (1:200, Cell Signaling Technology, 9101L) These primary antibodies were diluted in a 1.5 % NGS blocking solution with 0.1 % Triton X-100, 0.05 % Tween-20, and 1 % BSA. .. Following the primary antibody incubation, the sections were rinsed in PBS and incubated with appropriate fluorescent secondary antibodies, such as Alexa Fluor 647-conjugated goat anti-mouse (1:100, Invitrogen, A21235), Alexa Fluor 647-conjugated goat anti-rabbit (1:250, Invitrogen, A21244), or goat anti-rat Cy3 (1:250, Invitrogen, A10522) in a 1.5 % NGS blocking solution with 0.1% Triton X-100, 0.05 % Tween-20, and 1 % BSA.

Article Title: Neuronal Correlates of Hyperalgesia and Somatic Signs of Heroin Withdrawal in Male and Female Mice
Article Snippet: .. We incubated the sections overnight at 4°C with rabbit anti-phospho-c-Fos (1:2000; catalog #5348; Cell Signaling Technology) in blocking solution. .. After rinsing with PB three times for 10min each, we incubated the sections for 1 h at room temperature with biotinylated goat anti-rabbit antibody (1:200; catalog #PK6101; Vector Laboratories) in blocking solution.

Article Title: An accessory prefrontal cortex–thalamus circuit sculpts maternal behavior in virgin female mice
Article Snippet: Fixed brains were incubated in 30% sucrose for 48 h, frozen, embedded in OCT, and cut into free-floating 30–40 lm slices on a cryostat (Leica, Germany). .. A 1-in-6 series of slices were incubated with primary antibodies for rabbit anti-phospho-c-Fos (1:2,000; Cell Signaling Technology, 5348S), chicken anti-GFP (1: 500; Abcam, ab13970), and mouse anti-NeuN (1:1,000; Chemicon/SigmaAldrich, MAB377). .. Secondary antibodies are as follow: donkey antirabbit Alexa 647 (1:500, Invitrogen, A32795), goat anti-chicken Alexa 488 (1:500, Invitrogen, A32931), and goat anti-mouse CF 594 (1:500, Merck, SAB4600402).

Article Title: Cortical hyperexcitability drives dying forward amyotrophic lateral sclerosis symptoms and pathology in mice.
Article Snippet: .. Sections were then incubated overnight in primary antibodies; rat antiCOUP-TF-interacting protein 2 (CTIP2, 1:500, ab18465, Abcam), rabbit anti-phospho-c-Fos (1:200, 25348, Cell Signalling) and chicken anti-red fluorescent protein (RFP, 1:1000; 600–901–379S, Rockland). .. Following washes and blocking (described above), tissue was incubated in secondary antibodies; donkey anti-rabbit Alexa-Fluor 647 (1:400, 711–545–152, Jackson ImmunoResearch), donkey anti-rat Alexa-Fluor 488 (1:400, 712–605–153, Jackson ImmunoResearch) and donkey antichicken Alexa-Fluor 594 (1:400; 703–585–155, Jackson ImmunoResearch) as described above.

Next-Generation Sequencing:

Article Title: An inhibitory circuit from central amygdala to zona incerta drives pain-related behaviors in mice
Article Snippet: Sections were rinsed in PBS, incubated in 0.1% Triton X-100 in PBS for 10 min at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100, 0.05% Tween-20 and 1% bovine serum albumin (BSA) for 30 min at room temperature. .. Sections were then incubated for 72 hr at 4 °C in mouse anti-PKCδ (1:1000, BD Biosciences, 610397), rabbit anti-Phospho-c-Fos (1:2000, Cell Signaling Technology, 5348) or rat anti-mCherry (1:500, Invitrogen, M11217) in 1.5% NGS blocking solution with 0.1% Triton X-100, 0.05% Tween-20 and 1% BSA. .. Sections were then rinsed in PBS and incubated in Alexa Fluor 647-conjugated goat anti-mouse (1:100, Invitrogen, A21235), Alexa Fluor 647-conjugated goat anti-rabbit (1:250, Invitrogen, A21244), or goat anti-rat Cy3 (1:250, Invitrogen, A10522) secondary antibodies in 1.5% NGS blocking solution with 0.1% Triton X-100, 0.05% Tween 20 and 1% BSA, protected from light, for 2 hr at room temperature.

Blocking Assay:

Article Title: An inhibitory circuit from central amygdala to zona incerta drives pain-related behaviors in mice
Article Snippet: Sections were rinsed in PBS, incubated in 0.1% Triton X-100 in PBS for 10 min at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100, 0.05% Tween-20 and 1% bovine serum albumin (BSA) for 30 min at room temperature. .. Sections were then incubated for 72 hr at 4 °C in mouse anti-PKCδ (1:1000, BD Biosciences, 610397), rabbit anti-Phospho-c-Fos (1:2000, Cell Signaling Technology, 5348) or rat anti-mCherry (1:500, Invitrogen, M11217) in 1.5% NGS blocking solution with 0.1% Triton X-100, 0.05% Tween-20 and 1% BSA. .. Sections were then rinsed in PBS and incubated in Alexa Fluor 647-conjugated goat anti-mouse (1:100, Invitrogen, A21235), Alexa Fluor 647-conjugated goat anti-rabbit (1:250, Invitrogen, A21244), or goat anti-rat Cy3 (1:250, Invitrogen, A10522) secondary antibodies in 1.5% NGS blocking solution with 0.1% Triton X-100, 0.05% Tween 20 and 1% BSA, protected from light, for 2 hr at room temperature.

Article Title: Neuronal Correlates of Hyperalgesia and Somatic Signs of Heroin Withdrawal in Male and Female Mice
Article Snippet: .. We incubated the sections overnight at 4°C with rabbit anti-phospho-c-Fos (1:2000; catalog #5348; Cell Signaling Technology) in blocking solution. .. After rinsing with PB three times for 10min each, we incubated the sections for 1 h at room temperature with biotinylated goat anti-rabbit antibody (1:200; catalog #PK6101; Vector Laboratories) in blocking solution.

other:

Article Title: A genome-scale screen identifies sulfated glycosaminoglycans as pivotal in epithelial cell damage by Candida albicans
Article Snippet: Rabbit anti-phospho-c-Fos (Ser32, #5348), rabbit anti-phospho-EGFR (Tyr1068, #2234), rabbit anti-EGFR (#4267), rabbit anti-phospho-ERK1/2 (Thr202/Tyr204, #4370), rabbit anti-ERK1/2 (#4695), rabbit anti-GAPDH (#5174) were from Cell Signaling.

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